For each plot type, one can use the mouse to estimate enzyme kinetic parameters such as Vmax and Km. Plot types include (1) reaction velocity versus substrate concentration (Vo vs. [S]), (2) Lineweaver-Burk (1/Vo vs. [S]), (3) Eadie-Hofstee (Vo vs. Vo/[S]), (4) enzyme activity versus temperature (Vo vs. T), and (5) enzyme activity versus pH (Vo vs. pH).